Journal: STAR Protocols

Article Title: Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

doi: 10.1016/j.xpro.2020.100203

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Article Snippet: LPS from Salmonella Minnesota , Invivo Gen , Cat #tlrl-smlps.

Techniques: Recombinant, Microscopy, Adhesive, Isolation, Cell Isolation, Software

Journal: iScience

Article Title: Endothelial cells are a key target of IFN-g during response to combined PD-1/CTLA-4 ICB treatment in a mouse model of bladder cancer

doi: 10.1016/j.isci.2023.107937

Figure Lengend Snippet: Experimental design for single cell RNA and bulk DNA sequencing (A) Timelines for generating tumor samples for individual mice for each condition. (B) Workflow for generating single cell suspensions for single cell RNA sequencing for one of five biological replicates sequenced. For each condition in each replicate, tumors from three individual mice were pooled into one suspension and used to create libraries for 10x Genomics 5′ single cell gene expression (GEX), B cell receptor (BCR), and T cell receptor (TCR) sequencing. (C) Sources for normal and tumor bulk DNA sequencing. DNA was isolated from a normal mouse tail sample and an MCB6C tumor cell line sample for whole genome sequencing (WGS) and whole exome sequencing (WES). ICB = combined PD-1/CTLA-4 immune checkpoint blockade treatment, ICBΔT = combined PD-1/CTLA-4 immune checkpoint blockade treatment received after CD4 + T cell depletion, Isotype control = rat IgG2a and mouse IgG2b.

Article Snippet: InVivo MAb rat IgG2a isotype control, anti-trinitrophenol , BioXcell , Cat# BE0089, clone 2A3; RRID: AB_1107769.

Techniques: DNA Sequencing, RNA Sequencing Assay, Suspension, Expressing, Sequencing, Isolation

Journal: iScience

Article Title: Endothelial cells are a key target of IFN-g during response to combined PD-1/CTLA-4 ICB treatment in a mouse model of bladder cancer

doi: 10.1016/j.isci.2023.107937

Figure Lengend Snippet: Functional analysis confirms endothelial cells are a principal target of IFN-g and a key mediator of treatment response (A) Tumor diameter measurements for IFNgR1 intact and endothelial IFNgR1 knockout mice with and without ICB treatment over time (pre- and post-treatment). For all comparisons, a 2-way ANOVA for repeated measures was performed. P-values <0.05 were considered significant. Error bars represent one standard deviation. (B) Bar graphs displaying the percentage of CD4 + lymphocytes in the tumor microenvironment across the same four conditions as (A). (C) Bar graphs displaying the percentage of Tbet+, IFNg+ cells detected within the CD4 + T lymphocyte population across the same four conditions as (A). For all bar graphs, bar height indicates the average percentage across all mice from the given condition. Each point represents the percentage for an individual mouse. For all comparisons, a two-tailed, unpaired Student's t-test was performed. P-values <0.05 were considered significant. Error bars represent one standard deviation. f/f = flox/flox, ICB = combined PD-1/CTLA-4 immune checkpoint blockade treatment, Iso = rat IgG2a and mouse IgG2b isotype control. See also Figures S8–S10 and Table S8 .

Article Snippet: InVivo MAb rat IgG2a isotype control, anti-trinitrophenol , BioXcell , Cat# BE0089, clone 2A3; RRID: AB_1107769.

Techniques: Functional Assay, Knock-Out, Standard Deviation, Two Tailed Test

Journal: iScience

Article Title: Endothelial cells are a key target of IFN-g during response to combined PD-1/CTLA-4 ICB treatment in a mouse model of bladder cancer

doi: 10.1016/j.isci.2023.107937

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Article Snippet: InVivo MAb rat IgG2a isotype control, anti-trinitrophenol , BioXcell , Cat# BE0089, clone 2A3; RRID: AB_1107769.

Techniques: Purification, Software

Journal: iScience

Article Title: Heightened innate immune state induced by viral vector leads to enhanced response to challenge and prolongs malaria vaccine protection

doi: 10.1016/j.isci.2024.111468

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Article Snippet: InVivo MAb anti-mouse IL-18 , BioXCell , Cat#BE0237; RRID: AB_2687719.

Techniques: Control, Virus, Recombinant, Giemsa Stain, Cell Stimulation, Transfection, Software